The two-days quantitative real-time PCR workshop will provide attendees a comprehensive overview of real-time PCR and consists of a theoretical and practical part. The course contents are: • Theory of quantitative real time PCR (traditional PCR vs qPCR, detection methods, assay design, amplification plots, Cq, melting curves, efficiency, reference genes..) • Study design • Sample preparation and quality control (RNA isolation methods and RNA integrity, cDNA synthesis, qPCR reaction, assay validation, controls) • Relative quantification methods (ΔΔ Ct-method, Pfaffl-method) • Hands on training (SYBR Assays) • Troubleshooting Target audience: PhD students and technicians (qPCR beginners) Costs: 400 Euro (internal) / 600 Euro (external) Registration: firstname.lastname@example.org (Deadline: March 30th , 2018) As the number of participants is limited (min. 4 and max. 8), please register early to confirm your seat!
DFP Punkte: 12
Veranstaltungsort: Seminarraum 03 12, ZMF, Stiftingtalstraße 24, 8010 Graz
Birgit Galle, Ingeborg Klymiuk