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Medizinische Universität Graz

MEDICAL RESEARCH ACADEMY GRAZ
HIGH CONTENT SCREENING FOR MULTIPARAMETER PHENOTYPIC PROFILING OF CELLS - STAINING, IMAGE ACQUISITION AND STATISTICAL ANALYSIS OF DATA

 

High-Content Screening (HCS) uses automated microscopy, multi-parameter image processing, and visualization tools to extract quantitative data from cell populations and, thereby, allows a detailed analysis of individual cells regarding a variety of parameters. Compared to plate readers information on individual cells can be generated. The huge amount of data, which is generated, needs proper data management and statistic analysis for correct interpretation of data.

Theory: principle of common cell-based assays (cytotoxicity, apoptosis, oxidative stress, proliferation, organelle function) and of HCS, statistical methods.

Practical part: plate layout, seeding, treatment of plates for photometric and HCS analysis, MTS and cell staining, HCS data acquisition, statistical analysis of data

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