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Medizinische Universität Graz

MEDICAL RESEARCH ACADEMY GRAZ
REAL TIME PCR WORKSHOP

 

The two-days quantitative real-time PCR workshop will provide attendees a comprehensive overview of real-time PCR and consists of a theoretical and practical part.

The course contents are:

 

  • Theory of quantitative real time PCR (traditional PCR vs qPCR, detection methods, assay design, amplification plots, Cq, melting curves, efficiency, reference genes..)
  • Study design
  • Sample preparation and quality control (RNA isolation methods and QC, cDNA synthesis, qPCR reaction, assay validation, controls)
  • Relative quantification methods (∆∆ Ct-method, Pfaffl-method)
  • Hands on training (SYBR Assays)
  • Troubleshooting

 

For registration please fill in the registration form (Link or registration button on the left side)

 

 

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