The two-days quantitative real-time PCR workshop will provide attendees a comprehensive overview of real-time PCR and consists of a theoretical and practical part.

The course contents are:

• Theory of quantitative real time PCR (traditional PCR vs qPCR, detection methods, assay design, amplification plots, Cq, melting curves, efficiency, reference genes..)
• Study design
• Sample preparation and quality control (RNA isolation methods and QC, cDNA synthesis, qPCR reaction, assay validation, controls)
• Relative quantification methods (∆∆ Ct-method, Pfaffl-method)
• Hands on training (SYBR Assays)
• Troubleshooting

For registration please fill in the registration form (Link or registration button on the left side)