The course contents are:
•Theory of quantitative real time PCR (traditional PCR vs qPCR, detection methods, assay design, amplification plots, Cq, melting curves, efficiency, reference genes..)
•Sample preparation and quality control (RNA isolation methods and QC, cDNA synthesis, qPCR reaction, assay validation, controls)
•Relative quantification methods (ΔΔ Ct-method, Pfaffl-method)
•Hands on training (SYBR Assays)
•Troubleshooting Languages of instruction: English or German Target audience: qPCR beginners (PhD students, technicians and researchers) Costs: 400 Euro (University) / 600 Euro (Company) Registration: firstname.lastname@example.org (deadline: October, 31st 2018) As the number of participants is limited (min. 3 and max. 8), please register early to confirm your seat!
DFP Punkte: 16
Veranstaltungsort: ZMF, Seminarraum EG086